5WO6
Crystal Structure of Transient Receptor Potential (TRP) channel TRPV6cryst
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 145.621, 145.621, 114.136 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.915 - 3.310 |
R-factor | 0.2888 |
Rwork | 0.287 |
R-free | 0.31430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iwp |
RMSD bond length | 0.002 |
RMSD bond angle | 0.594 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 17758 |
<I/σ(I)> | 1.1 |
Completeness [%] | 99.8 |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 150mM NaCl, 100mM TRIS, 22% PEG 350 |