5WNK
Crystal structure of murine receptor-interacting protein 4 (Ripk4) D143N bound to TG100-115
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 69.769, 110.231, 146.277 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 3.110 |
R-factor | 0.22771 |
Rwork | 0.225 |
R-free | 0.27248 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.219 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.010 |
High resolution limit [Å] | 3.110 |
Rmerge | 0.014 |
Number of reflections | 16445 |
<I/σ(I)> | 9.4 |
Completeness [%] | 98.3 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 292 | 0.1M HEPES pH 7.5, 200 mM magnesium chloride, 10% isopropanol, 15% ethylene glycol, 10% PEG 4000 |