5WMK
Arabidopsis thaliana Prephenate Aminotransferase double mutant- T84V K169V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-12-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.498, 64.891, 51.933 |
| Unit cell angles | 90.00, 105.87, 90.00 |
Refinement procedure
| Resolution | 28.015 - 1.398 |
| R-factor | 0.1614 |
| Rwork | 0.161 |
| R-free | 0.17760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wmh |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.807 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.020 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.068 | 0.581 |
| Number of reflections | 73134 | 3572 |
| <I/σ(I)> | 27.33 | 1.49 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 25% (w/v) PEG-1500, 100 mM MIB buffer (sodium malonate dibasic monohydrate, imidazole, boric acid), pH 5.0 |
