5WM9
Crystal Structure of TetR family regulator Rv0078 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 224.909, 67.075, 56.016 |
| Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
| Resolution | 36.378 - 1.850 |
| R-factor | 0.209026281237 |
| Rwork | 0.207 |
| R-free | 0.24032 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.672 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.010 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.051 | 0.421 |
| Rpim | 0.031 | 0.261 |
| Number of reflections | 67749 | 9693 |
| <I/σ(I)> | 16.9 | 3 |
| Completeness [%] | 96.5 | 95.1 |
| Redundancy | 3.7 | 3.5 |
| CC(1/2) | 0.999 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 0.1 M sodium cacodylate, pH 6.4, 1.3 M lithium sulfate |
