5WLZ

Crystal Structure of Amino Acids 1677-1758 of Human Beta Cardiac Myosin Fused to Xrcc4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	85.290, 102.940, 136.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.068 - 3.500
R-factor	0.2154
Rwork	0.213
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ik9
RMSD bond length	0.002
RMSD bond angle	0.423
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.139	0.139
Number of reflections	16299	808
<I/σ(I)>	17.1	3.2
Completeness [%]	100.0	100
Redundancy	9.5	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	8-10% methyl-ether polyethylene glycol (MEPEG) 5K, 300 mM glycine, bis-tris propane pH 7.0, 1.5-3.0% (w/v) jeffamine M-600