5WLN
Cryo-EM structure of the T2SS secretin XcpQ from Pseudomonas aeruginosa
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.57 |
| Number of Particle | 18005 |
| Software | cryoSPARC,CTFFIND,NAMD,PHENIX |
| Symmetry | C15 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
