5WLF
Crystal structure of the apo PPS PHD finger
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-07 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.27 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.020, 41.060, 26.410 |
| Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
| Resolution | 26.124 - 1.400 |
| R-factor | 0.1381 |
| Rwork | 0.136 |
| R-free | 0.16020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l7x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.882 |
| Data reduction software | d*TREK |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.124 |
| High resolution limit [Å] | 1.200 |
| Number of reflections | 9642 |
| <I/σ(I)> | 53.02 |
| Completeness [%] | 80.1 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M HEPES pH 7.5, 0.2 M NaCl, 25% PEG 3,000 |
