5WLE
Crystal structure of the PPS PHD finger in complex with H3K4me3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 22.972, 44.223, 31.308 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 31.200 - 1.952 |
| R-factor | 0.1492 |
| Rwork | 0.145 |
| R-free | 0.18620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l7x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.877 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.200 |
| High resolution limit [Å] | 1.952 |
| Number of reflections | 4556 |
| <I/σ(I)> | 37.9 |
| Completeness [%] | 98.7 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277.15 | 0.1 mM Tris and 24% PEG 6,000 |
