5WL2
VH1-69 germline antibody with CDR H3 sequence of CR9114
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 77.2 |
Detector technology | PIXEL |
Collection date | 2017-02-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97946 |
Spacegroup name | P 32 |
Unit cell lengths | 71.125, 71.125, 185.705 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.663 - 2.000 |
R-factor | 0.2017 |
Rwork | 0.200 |
R-free | 0.23560 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.111 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.663 | 1.960 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.060 | |
Rpim | 0.030 | 0.430 |
Number of reflections | 81962 | 4107 |
<I/σ(I)> | 11.5 | 1.3 |
Completeness [%] | 98.9 | 97.3 |
Redundancy | 4.5 | 3.8 |
CC(1/2) | 1.000 | 0.610 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.095 M sodium citrate pH 5.6 19% 2-propanol 5% (v/v) glycerol 19% (w/v) PEG4000 |