5WKY
Bromide sites in the structure of an acid sensing ion channel in a resting state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9191 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.550, 134.490, 159.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.150 - 4.000 |
R-factor | 0.2817 |
Rwork | 0.281 |
R-free | 0.29890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wku |
RMSD bond length | 0.004 |
RMSD bond angle | 0.706 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2597: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.000 |
High resolution limit [Å] | 4.000 |
Number of reflections | 38333 |
<I/σ(I)> | 9.46 |
Completeness [%] | 99.9 |
Redundancy | 7 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100 mM Tris pH 8.5, 100 mM NaCl, 29% PEG 400, 5 mM BaCl2. Crystal soaked in 150 mM NaBr prior to freezing. |