5WKW
Crystal structure of apo wild type peptidylglycine alpha-hydroxylating monooxygenase (PHM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-22 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.726, 66.568, 70.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.300 - 1.790 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.27350 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | pdbid XX |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.810 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.820 |
| High resolution limit [Å] | 1.790 | 4.860 | 1.790 |
| Rmerge | 0.078 | 0.055 | 0.387 |
| Total number of observations | 195741 | ||
| Number of reflections | 26454 | 1459 | 1193 |
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 99.1 | 99.3 | 92 |
| Redundancy | 7.4 | 10.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 19-24% PEG 4000, Tris HCL |
