5WKT
3.2-Angstrom In situ Mylar structure of bovine opsin at 100 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 242.043, 242.043, 110.637 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.740 - 3.200 |
R-factor | 0.2746 |
Rwork | 0.274 |
R-free | 0.29460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j4q |
RMSD bond length | 0.007 |
RMSD bond angle | 0.875 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.740 |
High resolution limit [Å] | 3.200 |
Rmerge | 0.075 |
Number of reflections | 20539 |
<I/σ(I)> | 9.23 |
Completeness [%] | 100.0 |
Redundancy | 2 |
CC(1/2) | 0.970 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 280 | 100 mM sodium acetate , 3.8 M (NH4)2SO4, ~12% (w/v) trehalose |