5WKN

Crystal structure of the parainfluenza virus 5 nucleoprotein-phosphoprotein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 2 21 21
Unit cell lengths	79.087, 81.063, 152.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.180 - 2.653
R-factor	0.2533
Rwork	0.251
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4co6
RMSD bond length	0.005
RMSD bond angle	0.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MrBUMP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.079
Rpim	0.031	0.462
Number of reflections	29196	1433
<I/σ(I)>	30.3	1.78
Completeness [%]	99.9	100
Redundancy	7.3	7.5
CC(1/2)		0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M CaCl2, 0.1 M HEPES sodium pH 7.0 and 24 % (v/v) PEG 400