5WKD

Crystal structure of the segment, GNNQGSN, from the low complexity domain of TDP-43, residues 300-306

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	50.347, 4.777, 14.746
Unit cell angles	90.00, 101.73, 90.00

Refinement procedure

Resolution	24.650 - 1.800
R-factor	0.1843
Rwork	0.184
R-free	0.19490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	idealized beta strand
RMSD bond length	0.021
RMSD bond angle	1.804
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.158	0.075	0.250
Total number of observations	845
Number of reflections	373	45	28
<I/σ(I)>	5.4
Completeness [%]	91.6	90	84.8
Redundancy	2.3	2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100mM Bis-Tris propane, pH 7.5, 200 mM sodium sulfate, 20% PEG3350