5WJY
Cryo-EM structure of B. subtilis flagellar filaments S285P
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Staining | Yes |
Instrument | FEI VITROBOT MARK IV |
Staining material | negative stain |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 4.5 |
Software | EMAN2,SPIDER,PHENIX,Coot,EPU,CTFFIND3,Rosetta |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |