5WJT
Cryo-EM structure of B. subtilis flagellar filaments N226Y
EM specimen
| Vitrification | Yes |
| Staining | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Staining material | negative stain |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.8 |
| Software | EMAN2,Coot,SPIDER,EPU,CTFFIND3,Rosetta,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
