5WJR
High resolution native hexamer DNA and RNA hybrid in complex with RNase H catalytic domain D132N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.264, 37.644, 62.121 |
| Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
| Resolution | 61.790 - 1.700 |
| R-factor | 0.19098 |
| Rwork | 0.189 |
| R-free | 0.23394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8u |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.849 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.790 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.033 | 0.136 |
| Number of reflections | 20483 | 1081 |
| <I/σ(I)> | 21.1 | 3.5 |
| Completeness [%] | 92.1 | 53.7 |
| Redundancy | 5.8 | 3.3 |
| CC(1/2) | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES pH 6.5, 12%(w/v) PEG 20000 |
