5WJQ

mouseZFP568-ZnF2-11 in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-18
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	180.641, 35.239, 81.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.865 - 2.794
R-factor	0.1963
Rwork	0.193
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v3m
RMSD bond length	0.008
RMSD bond angle	1.039
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.900	2.890
High resolution limit [Å]	2.790	2.790
Rmerge	0.132	0.956
Number of reflections	13731	1328
<I/σ(I)>	17.6	4.5
Completeness [%]	99.9	100
Redundancy	13.7	13.5
CC(1/2)	0.933	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		286	0.2 M calcium acetate 16% polyethylene glycol 3350