5WJG
Using sound pulses to solve the crystal harvesting bottleneck
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97913 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.041, 68.041, 102.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 102.290 - 1.500 |
R-factor | 0.12528 |
Rwork | 0.124 |
R-free | 0.15736 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fon |
RMSD bond length | 0.025 |
RMSD bond angle | 2.208 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 102.290 |
High resolution limit [Å] | 1.500 |
Rmerge | 0.076 |
Number of reflections | 36472 |
<I/σ(I)> | 21.8 |
Completeness [%] | 97.9 |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6.5 | 295 | 0.1 M BisTris pH 6.5, 0.08M CaCl2 |