5WJ7
Crystal Structure of Amino Acids 1733-1797 of Human Beta Cardiac Myosin Fused to Xrcc4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.032, 71.506, 173.056 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.243 - 2.500 |
R-factor | 0.2058 |
Rwork | 0.203 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ik9 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.372 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.470 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.044 | 0.185 |
Number of reflections | 21429 | 1036 |
<I/σ(I)> | 53.6 | 7.86 |
Completeness [%] | 99.6 | 97.2 |
Redundancy | 12 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 16% (w/v) Methyl-Ether PEG 5K, 300 mM glycine, 100 mM triethanolamine pH 7.5 |