5WJ2
Crystal structure of the green fluorescent protein Clover
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.063, 135.176, 168.589 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.147 - 2.409 |
R-factor | 0.1943 |
Rwork | 0.193 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qyq |
RMSD bond length | 0.007 |
RMSD bond angle | 0.931 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmerge | 0.116 | 0.049 | 0.843 |
Rmeas | 0.124 | 0.053 | 0.907 |
Rpim | 0.045 | 0.020 | 0.333 |
Number of reflections | 23899 | ||
<I/σ(I)> | 10.1 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 7.4 | 6.8 | 7.4 |
CC(1/2) | 0.998 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 100mM Tris, pH 8.5, 50mM MgCl2, 25% PEG3350 |