5WIA
Crystal structure of the segment, GNNSYS, from the low complexity domain of TDP-43, residues 370-375
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 4.786, 15.617, 40.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.340 - 1.002 |
| R-factor | 0.1574 |
| Rwork | 0.155 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ideal 5-residue polyalanine beta-strand |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.183 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 2.150 | 1.000 |
| Rmerge | 0.155 | 0.060 | 0.554 |
| Rmeas | 0.172 | 0.067 | 0.714 |
| Rpim | 0.072 | 0.029 | 0.443 |
| Total number of observations | 10032 | ||
| Number of reflections | 1864 | 111 | |
| <I/σ(I)> | 5.2 | ||
| Completeness [%] | 95.2 | 99.6 | 62.7 |
| Redundancy | 5.4 | 5.3 | 1.9 |
| CC(1/2) | 0.997 | 0.368 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100mM bis tris propane 8.5, 200mM sodium nitrate, 20% PEG 3350 |
