5WI2
Crystal structure of the KA1 domain from human Chk1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 3 |
Unit cell lengths | 76.818, 76.818, 31.828 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.409 - 2.495 |
R-factor | 0.2166 |
Rwork | 0.214 |
R-free | 0.24450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ehb |
RMSD bond length | 0.006 |
RMSD bond angle | 0.737 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.495 |
Rmerge | 0.097 |
Number of reflections | 7313 |
<I/σ(I)> | 24.5 |
Completeness [%] | 99.9 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | Sodium Acetate Trihydrate, Ammonium Sulfate |