Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.002, 55.275, 67.942 |
| Unit cell angles | 107.44, 98.91, 103.23 |
Refinement procedure
| Resolution | 32.410 - 1.690 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t9e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.410 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.057 | 0.510 |
| Number of reflections | 64687 | 3130 |
| <I/σ(I)> | 13.77 | 1.1 |
| Completeness [%] | 97.0 | 94.6 |
| Redundancy | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% PEG-4000 AND 100 MM SODIUM CITRATE, PH 5.5 WITH 30% (W/V) D-SORBITOL AS AN ADDITIVE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K |
