5WHT
Crystal structure of 3'SL bound PltB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-28 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.379, 95.682, 117.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.437 - 1.932 |
| R-factor | 0.1627 |
| Rwork | 0.160 |
| R-free | 0.21120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rhr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.080 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.930 |
| Number of reflections | 51433 |
| <I/σ(I)> | 14.41 |
| Completeness [%] | 99.6 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 297 | PEG 1500 |
