5WHN

Crystal structure of the segment, NFGAFS, from the low complexity domain of TDP-43, residues 312-317

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	13.820, 4.850, 46.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.253 - 1.100
R-factor	0.136
Rwork	0.133
R-free	0.16010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dgj
RMSD bond length	0.006
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_1555)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	13.253	13.253	1.140
High resolution limit [Å]	1.100	4.110	1.100
Rmerge	0.172	0.094	0.726
Rmeas	0.184	0.103	0.789
Total number of observations	12008
Number of reflections	1447	38	123
<I/σ(I)>	9.65	15.95	2.52
Completeness [%]	94.0	92.7	75.9
Redundancy	8.299	5.763	6.374
CC(1/2)	0.991	0.989	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	100mM phosphate/citrate 4.2, 40% ethanol, 5% PEG 1000