5WHJ

Crystal structure of Fab fragment of anti-FcRn antibody DX-2507

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 43 2 2
Unit cell lengths	116.170, 116.170, 79.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.072 - 2.150
R-factor	0.1821
Rwork	0.180
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3idx
RMSD bond length	0.007
RMSD bond angle	0.838
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2863))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.057	0.589
Rmeas	0.061	0.022
Total number of observations	218958
Number of reflections	29879	378
<I/σ(I)>	23.8	3.8
Completeness [%]	99.3	100
Redundancy	7.3	7.4
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	DX-2507 Fab at 9.5 mg/mL against Morpheus screen condition G5 with 10% PEG 20000, 20% PEG 550