5WHI
Crystal Structure of Bcl-2-related protein A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.510, 43.090, 43.440 |
| Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
| Resolution | 30.120 - 1.690 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i1h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.863 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.120 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmeas | 0.050 | 1.297 |
| Rpim | 0.027 | 0.708 |
| Total number of observations | 51640 | 2693 |
| Number of reflections | 15715 | |
| <I/σ(I)> | 13.6 | 1.1 |
| Completeness [%] | 98.1 | 98.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG2000 |
