5WFX
Structural basis for the interaction of 14-3-3beta with Tricarboxylic Acid Cycle intermediate Malate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.930, 87.790, 124.044 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.687 - 1.651 |
R-factor | 0.2016 |
Rwork | 0.200 |
R-free | 0.23080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wfu |
RMSD bond length | 0.006 |
RMSD bond angle | 0.804 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.650 |
Rmerge | 0.060 |
Number of reflections | 78578 |
<I/σ(I)> | 36.3 |
Completeness [%] | 99.3 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 291 | 2.2M Sodium Malonate pH7.0 |