5WFT
PelB 319-436 from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.140, 61.140, 217.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.776 - 2.821 |
| R-factor | 0.2208 |
| Rwork | 0.215 |
| R-free | 0.27180 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.094 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.820 |
| Rmerge | 0.072 |
| Number of reflections | 10961 |
| <I/σ(I)> | 15.6 |
| Completeness [%] | 99.8 |
| Redundancy | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 HEPES pH 7.5, 13% (w/v) PEG 3350, 0.2 M proline |
