5WFT

PelB 319-436 from Pseudomonas aeruginosa PAO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-05
Detector	RAYONIX MX300HE
Wavelength(s)	0.9786
Spacegroup name	P 65 2 2
Unit cell lengths	61.140, 61.140, 217.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.776 - 2.821
R-factor	0.2208
Rwork	0.215
R-free	0.27180
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.094
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.820
Rmerge	0.072
Number of reflections	10961
<I/σ(I)>	15.6
Completeness [%]	99.8
Redundancy	19.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 HEPES pH 7.5, 13% (w/v) PEG 3350, 0.2 M proline