5WFG
Crystal structure of the TarA wall teichoic acid glycosyltransferase bound to UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.930, 104.210, 90.130 |
Unit cell angles | 90.00, 108.27, 90.00 |
Refinement procedure
Resolution | 33.030 - 2.900 |
R-factor | 0.204 |
Rwork | 0.200 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.6.1) |
Refinement software | BUSTER |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.030 | 33.030 | 2.970 |
High resolution limit [Å] | 2.890 | 12.930 | 2.890 |
Rmerge | 0.074 | 0.020 | 0.646 |
Rmeas | 0.105 | 0.028 | 0.911 |
Number of reflections | 44606 | 477 | 2987 |
<I/σ(I)> | 7.23 | 26.6 | 1.04 |
Completeness [%] | 88.7 | 86.1 | 79.1 |
Redundancy | 1.601 | 1.616 | 1.567 |
CC(1/2) | 0.995 | 0.998 | 0.594 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 40% PEG-300, 200mM calcium acetate hydrate, 100mM sodium cacodylate |