5WFB
Tepsin tENTH domain 1-136.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-10-30 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 80.870, 84.750, 80.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.821 - 1.382 |
R-factor | 0.1577 |
Rwork | 0.156 |
R-free | 0.18530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | basic alpha helical model |
RMSD bond length | 0.012 |
RMSD bond angle | 1.051 |
Data reduction software | MOSFLM |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.821 |
High resolution limit [Å] | 1.380 |
Number of reflections | 56820 |
<I/σ(I)> | 14.6 |
Completeness [%] | 99.6 |
Redundancy | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 12% (w/v) PEG3350, 4% (v/v) tacsimate pH 5.0 |