5WF9
Tepsin tENTH domain 1-153
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-10-27 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 90.180, 90.180, 42.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.090 - 1.800 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wfb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.645 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.800 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.103 | 1.905 |
| Rpim | 0.020 | 0.462 |
| Number of reflections | 16739 | |
| <I/σ(I)> | 24.9 | 1.5 |
| Completeness [%] | 98.9 | 91.6 |
| Redundancy | 26.8 | 17.4 |
| CC(1/2) | 1.000 | 0.534 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 150mM MES monohydrate pH 6.0, 15% (w/v) PEG6000. |
