5WEG
Crystal Structure of UDP-glucose pyrophosphorylase from Sugarcane
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 |
Unit cell lengths | 61.349, 66.789, 74.785 |
Unit cell angles | 75.62, 79.00, 77.52 |
Refinement procedure
Resolution | 42.734 - 2.000 |
R-factor | 0.1938 |
Rwork | 0.193 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z90 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.838 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.820 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.123 | 0.493 |
Number of reflections | 73174 | 4425 |
<I/σ(I)> | 5.6 | 2.1 |
Completeness [%] | 97.5 | 95 |
Redundancy | 2 | 1.9 |
CC(1/2) | 0.978 | 0.787 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | MES sodium salt, ammonium sulfate, PEG 8000 |