5WEG
Crystal Structure of UDP-glucose pyrophosphorylase from Sugarcane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.349, 66.789, 74.785 |
| Unit cell angles | 75.62, 79.00, 77.52 |
Refinement procedure
| Resolution | 42.734 - 2.000 |
| R-factor | 0.1938 |
| Rwork | 0.193 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z90 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.820 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.123 | 0.493 |
| Number of reflections | 73174 | 4425 |
| <I/σ(I)> | 5.6 | 2.1 |
| Completeness [%] | 97.5 | 95 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 0.978 | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | MES sodium salt, ammonium sulfate, PEG 8000 |
