5WEE
Crystal structure of HpVAL4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-08-15 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.560, 61.565, 74.819 |
| Unit cell angles | 111.59, 90.14, 113.47 |
Refinement procedure
| Resolution | 40.883 - 1.990 |
| R-factor | 0.1788 |
| Rwork | 0.176 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u53 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.974 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2722)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.700 |
| High resolution limit [Å] | 1.990 |
| Rmerge | 0.139 |
| Rpim | 0.057 |
| Number of reflections | 48537 |
| <I/σ(I)> | 18 |
| Completeness [%] | 95.4 |
| Redundancy | 7.2 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M NaAcetate pH 4.5, 22.5% Poly PEG 0.3 -8kD |
