5WE1
Structural Basis for Shelterin Bridge Assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977408 |
| Spacegroup name | P 31 |
| Unit cell lengths | 66.110, 66.110, 123.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.917 - 3.202 |
| R-factor | 0.2691 |
| Rwork | 0.266 |
| R-free | 0.31650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5we2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.269 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.250 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.104 | 0.765 |
| Number of reflections | 9911 | |
| <I/σ(I)> | 11.5 | 2.6 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris-HCl pH 8.0, 4% Reagent Alcohol, 0.3 M MgCl2 |
