5WE1

Structural Basis for Shelterin Bridge Assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977408
Spacegroup name	P 31
Unit cell lengths	66.110, 66.110, 123.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.917 - 3.202
R-factor	0.2691
Rwork	0.266
R-free	0.31650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5we2
RMSD bond length	0.005
RMSD bond angle	1.269
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.250	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.104	0.765
Number of reflections	9911
<I/σ(I)>	11.5	2.6
Completeness [%]	99.7	99.5
Redundancy	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Tris-HCl pH 8.0, 4% Reagent Alcohol, 0.3 M MgCl2