5WDS
Choanoflagellate Salpingoeca rosetta Ras with GDP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999981 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.222, 67.222, 184.679 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.530 - 1.850 |
R-factor | 0.2178 |
Rwork | 0.216 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5p21 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.885 |
Data reduction software | XDS (November 3, 2014) |
Data scaling software | Aimless (0.5.4) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.530 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.093 | 1.437 |
Rpim | 0.027 | 0.723 |
Number of reflections | 37417 | 2254 |
<I/σ(I)> | 12.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.9 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 20% PEG3350, 100 mM Tris-HCl, pH 9.0, 100 mM calcium chloride |