5WD8
Crystal structure of Legionella pneumophila effector lpg2328
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.044, 81.143, 45.684 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 40.572 - 1.944 |
| R-factor | 0.2186 |
| Rwork | 0.216 |
| R-free | 0.26990 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.334 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.575 | 1.980 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 12194 | 1083 |
| <I/σ(I)> | 19.9 | 2.5 |
| Completeness [%] | 99.1 | 91.6 |
| Redundancy | 6.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% (w/v) PEG 3350, 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5 |
