5WC6
Structure of a bacterial polysialyltransferase at 2.2 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-16 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.07252 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 78.255, 78.255, 301.995 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.670 - 2.200 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.670 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.766 |
| Number of reflections | 55737 | |
| <I/σ(I)> | 22 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 5.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 296 | 17% - 24% PEG3350 (v/v), 140 - 250 mM Mg2SO4 and 100 mM MES pH 7.2 |
