5WC3
SpoIIIAG
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Energy Filter | Gatan GIF |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.5 |
| Number of Particle | 770000 |
| Software | EMAN2,RELION,SerialEM,CTFFIND4 |
| Symmetry | C30 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
