5WC2

Crystal Structure of ADP-bound human TRIP13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	98.369, 98.369, 122.109
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.199 - 2.500
R-factor	0.1896
Rwork	0.188
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xgu
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.199	2.610
High resolution limit [Å]	2.500	2.500
Rmerge	0.079	1.490
Rpim	0.021	0.461
Number of reflections	23254	1349
<I/σ(I)>	40.6	2
Completeness [%]	100.0	100
Redundancy	15.2	10.9
CC(1/2)		0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	HEPES, sodium chloride, isopropanol