5WBJ

Crystal structure of the arabidopsis thaliana Raptor in complex with the TOS peptide of human 4EBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	89.100, 113.100, 153.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.2179
Rwork	0.215
R-free	0.26982
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wbi
RMSD bond length	0.008
RMSD bond angle	1.329
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.082	0.028	0.800
Rmeas	0.089	0.033	0.862
Rpim	0.035	0.015	0.307
Total number of observations	196625
Number of reflections	31652		3118
<I/σ(I)>	5.3
Completeness [%]	98.7	96.1	99.6
Redundancy	6.2	4.5	7.2
CC(1/2)		0.999	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	tacsimate