5WAX
Crystal Structure of Sugarcane SAPK10 (serine/threonine-protein kinase 10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-22 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.979200 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.420, 214.630, 93.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.000 |
| R-factor | 0.20038 |
| Rwork | 0.198 |
| R-free | 0.23676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zut |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.493 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.576 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 1.100 |
| Number of reflections | 51618 | 3763 |
| <I/σ(I)> | 15.7 | 1.6 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.5 | 5.6 |
| CC(1/2) | 0.990 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5M ammonium sulfate; 0.1M bis-tris pH 6.5; 0.1M sodium chloride. |
