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5WAI

Crystal Structure of a Suz12-Rbbp4-Jarid2-Aebp2 Heterotetrameric Complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL12-2
Synchrotron siteSSRL
BeamlineBL12-2
Temperature [K]93
Detector technologyPIXEL
Collection date2016-06-01
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.97946
Spacegroup nameP 21 21 21
Unit cell lengths95.300, 111.428, 253.254
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.100 - 2.900
R-factor0.1734
Rwork0.171
R-free0.21610
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3gfc
RMSD bond length0.010
RMSD bond angle1.200
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwareBUSTER (2.10.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.1002.950
High resolution limit [Å]2.8002.900
Rmerge0.1502.851
Rpim0.0420.828
Number of reflections675332989
<I/σ(I)>22.41.5
Completeness [%]99.199.8
Redundancy13.112.6
CC(1/2)0.9900.472
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP29310% PEG6000, 5%v/v(+/-)-2-Methyl-2,4-pentaneediol (MPD) and 100mM HEPES pH 7.5

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