5W94
Crystal structure of Scc4 in complex with Scc2n and Ctf19n
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-E |
Synchrotron site | APS |
Beamline | 21-ID-E |
Temperature [K] | 63 |
Detector technology | CCD |
Collection date | 2016-02-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97918 |
Spacegroup name | P 62 |
Unit cell lengths | 172.969, 172.969, 145.095 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.940 - 3.193 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xdn |
RMSD bond length | 0.011 |
RMSD bond angle | 1.416 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.940 | |
High resolution limit [Å] | 3.190 | 3.190 |
Number of reflections | 40688 | |
<I/σ(I)> | 24.37 | |
Completeness [%] | 99.1 | |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6 | 291 | 8% PEG4000, 80 mM PIPES, pH 6.0 |