5W93
p130Cas complex with paxillin LD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.290, 37.741, 152.502 |
Unit cell angles | 90.00, 100.94, 90.00 |
Refinement procedure
Resolution | 29.947 - 2.001 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3T6G chain B |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 31426 | 2436 |
<I/σ(I)> | 30.3 | 2.5 |
Completeness [%] | 97.3 | 77.1 |
Redundancy | 5.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 291 | The final 5 ul crystallization drop contained 0.5 mM protein and 2 mM peptide. The drop and 500 ul reservoir solution both contained 20 mM HEPES, pH 7.5, and 50 mM KCl |