5W8X
Lipid A Disaccharide Synthase (LpxB)-7 solubilizing mutations-Bound to UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 67.806, 67.806, 153.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.860 - 1.980 |
R-factor | 0.2006 |
Rwork | 0.199 |
R-free | 0.23090 |
Structure solution method | SAD |
Data reduction software | XDS (November 3, 2014) |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHENIX (v1.10_2155) |
Refinement software | PHENIX (v1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 153.880 | 2.030 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.063 | 1.272 |
Rpim | 0.035 | 0.753 |
Number of reflections | 29131 | 1983 |
<I/σ(I)> | 13.2 | |
Completeness [%] | 99.1 | 97.8 |
Redundancy | 4.1 | 3.7 |
CC(1/2) | 0.999 | 0.450 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 292 | 39% PEG 4000, 0.6 M LiCl, 0.1 M Tris-HCl pH 8.6 combined 1:1 with 8 g/L protein in 0.3 M NaCl, 5% glycerol, 20 mM Tris-HCl pH 7.4, 5 mM DTT with 100 mM trimethylammonium chloride used as an additive at 10 mM or 10% of drop volume |