5W8M

Crystal structure of Chaetomium thermophilum Vps29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	65.460, 106.740, 88.118
Unit cell angles	90.00, 95.45, 90.00

Refinement procedure

Resolution	45.594 - 1.520
R-factor	0.1553
Rwork	0.155
R-free	0.17660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w24
RMSD bond length	0.010
RMSD bond angle	1.296
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	1.550
High resolution limit [Å]	1.520	1.520
Rmerge	0.090	0.707
Rpim	0.057	0.431
Number of reflections	184667	8919
<I/σ(I)>	9.9	2.1
Completeness [%]	99.8	97.7
Redundancy	7.5	7.2
CC(1/2)	0.997	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	8% PEG20000, 8% PEG550MME, 0.2 M calcium acetate and 0.1 M Tris (pH 8.0)