5W8M
Crystal structure of Chaetomium thermophilum Vps29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.460, 106.740, 88.118 |
| Unit cell angles | 90.00, 95.45, 90.00 |
Refinement procedure
| Resolution | 45.594 - 1.520 |
| R-factor | 0.1553 |
| Rwork | 0.155 |
| R-free | 0.17660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w24 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.296 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.090 | 0.707 |
| Rpim | 0.057 | 0.431 |
| Number of reflections | 184667 | 8919 |
| <I/σ(I)> | 9.9 | 2.1 |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 7.5 | 7.2 |
| CC(1/2) | 0.997 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 8% PEG20000, 8% PEG550MME, 0.2 M calcium acetate and 0.1 M Tris (pH 8.0) |
