5W83
Rpn8/Rpn11 dimer complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.720, 74.990, 82.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.482 - 1.554 |
R-factor | 0.2143 |
Rwork | 0.213 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o8x |
RMSD bond length | 0.007 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.482 |
High resolution limit [Å] | 1.550 |
Number of reflections | 52965 |
<I/σ(I)> | 13.1 |
Completeness [%] | 99.0 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 1.5 M ammonium tartrate pH 6.8 |