5W7V
CryoEM structure of the segment, DLIIKGISVHI, assembled into a triple-helical fibril
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.8 |
| Software | EMAN,RELION,PHENIX,Leginon,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
